Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model
Identifieur interne : 000015 ( OpenAccess/Analysis ); précédent : 000014; suivant : 000016Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model
Auteurs : Yuka Tsumanuma ; Takanori Iwata ; Atsuhiro Kinoshita ; Kaoru Washio ; Toshiyuki Yoshida ; Azusa Yamada ; Ryo Takagi ; Masayuki Yamato ; Teruo Okano ; Yuichi IzumiSource :
- BioResearch Open Access [ 2164-7844 ] ; 2016.
Abstract
Periodontitis is a chronic inflammatory disease that induces the destruction of tooth-supporting tissues, followed by tooth loss. Although several approaches have been applied to periodontal regeneration, complete periodontal regeneration has not been accomplished. Tissue engineering using a combination of cells and scaffolds is considered to be a viable alternative strategy. We have shown that autologous transplantation of periodontal ligament-derived multipotent mesenchymal stromal cell (PDL-MSC) sheets regenerates periodontal tissue in canine models. However, the indications for autologous cell transplantation in clinical situations are limited. Therefore, this study evaluated the safety and efficacy of allogeneic transplantation of PDL-MSC sheets using a canine horizontal periodontal defect model. Canine PDL-MSCs were labeled with enhanced green fluorescent protein (EGFP) and were cultured on temperature-responsive dishes. Three-layered cell sheets were transplanted around denuded root surfaces either autologously or allogeneically. A mixture of β-tricalcium phosphate and collagen gel was placed on the bone defects. Eight weeks after transplantation, dogs were euthanized and subjected to microcomputed tomography and histological analyses. RNA and DNA were extracted from the paraffin sections to verify the presence of EGFP at the transplantation site. Inflammatory markers from peripheral blood sera were quantified using an enzyme-linked immunosorbent assay. Periodontal regeneration was observed in both the autologous and the allogeneic transplantation groups. The allogeneic transplantation group showed particularly significant regeneration of newly formed cementum, which is critical for the periodontal regeneration. Serum levels of inflammatory markers from peripheral blood sera showed little difference between the autologous and allogeneic groups. EGFP amplicons were detectable in the paraffin sections of the allogeneic group. These results suggest that allogeneic PDL-MSC sheets promoted periodontal tissue regeneration without side effects. Therefore, allogeneic transplantation of PDL-MSC sheets has a potential to become an alternative strategy for periodontal regeneration.
Url:
DOI: 10.1089/biores.2015.0043
PubMed: 26862470
PubMed Central: 4744877
Affiliations:
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Washio</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yoshida, Toshiyuki" sort="Yoshida, Toshiyuki" uniqKey="Yoshida T" first="Toshiyuki" last="Yoshida">Toshiyuki Yoshida</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yamada, Azusa" sort="Yamada, Azusa" uniqKey="Yamada A" first="Azusa" last="Yamada">Azusa Yamada</name><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Takagi, Ryo" sort="Takagi, Ryo" uniqKey="Takagi R" first="Ryo" last="Takagi">Ryo Takagi</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yamato, Masayuki" sort="Yamato, Masayuki" uniqKey="Yamato M" first="Masayuki" last="Yamato">Masayuki Yamato</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Okano, Teruo" sort="Okano, Teruo" uniqKey="Okano T" first="Teruo" last="Okano">Teruo Okano</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Izumi, Yuichi" sort="Izumi, Yuichi" uniqKey="Izumi Y" first="Yuichi" last="Izumi">Yuichi Izumi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26862470</idno><idno type="pmc">4744877</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744877</idno><idno type="RBID">PMC:4744877</idno><idno type="doi">10.1089/biores.2015.0043</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000086</idno><idno type="wicri:Area/Pmc/Curation">000086</idno><idno type="wicri:Area/Pmc/Checkpoint">000072</idno><idno type="wicri:Area/Ncbi/Merge">000407</idno><idno type="wicri:Area/Ncbi/Curation">000407</idno><idno type="wicri:Area/Ncbi/Checkpoint">000407</idno><idno type="wicri:doubleKey">2164-7844:2016:Tsumanuma Y:allogeneic:transplantation:of</idno><idno 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last="Kinoshita">Atsuhiro Kinoshita</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author><author><name sortKey="Washio, Kaoru" sort="Washio, Kaoru" uniqKey="Washio K" first="Kaoru" last="Washio">Kaoru Washio</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yoshida, Toshiyuki" sort="Yoshida, Toshiyuki" uniqKey="Yoshida T" first="Toshiyuki" last="Yoshida">Toshiyuki Yoshida</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yamada, Azusa" sort="Yamada, Azusa" uniqKey="Yamada A" first="Azusa" last="Yamada">Azusa Yamada</name><affiliation><nlm:aff id="aff4"></nlm:aff></affiliation></author><author><name sortKey="Takagi, Ryo" sort="Takagi, Ryo" uniqKey="Takagi R" first="Ryo" last="Takagi">Ryo Takagi</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Yamato, Masayuki" sort="Yamato, Masayuki" uniqKey="Yamato M" first="Masayuki" last="Yamato">Masayuki Yamato</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Okano, Teruo" sort="Okano, Teruo" uniqKey="Okano T" first="Teruo" last="Okano">Teruo Okano</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Izumi, Yuichi" sort="Izumi, Yuichi" uniqKey="Izumi Y" first="Yuichi" last="Izumi">Yuichi Izumi</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">BioResearch Open Access</title><idno type="ISSN">2164-7844</idno><idno type="eISSN">2164-7860</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Periodontitis is a chronic inflammatory disease that induces the destruction of tooth-supporting tissues, followed by tooth loss. Although several approaches have been applied to periodontal regeneration, complete periodontal regeneration has not been accomplished. Tissue engineering using a combination of cells and scaffolds is considered to be a viable alternative strategy. We have shown that autologous transplantation of periodontal ligament-derived multipotent mesenchymal stromal cell (PDL-MSC) sheets regenerates periodontal tissue in canine models. However, the indications for autologous cell transplantation in clinical situations are limited. Therefore, this study evaluated the safety and efficacy of allogeneic transplantation of PDL-MSC sheets using a canine horizontal periodontal defect model. Canine PDL-MSCs were labeled with enhanced green fluorescent protein (EGFP) and were cultured on temperature-responsive dishes. Three-layered cell sheets were transplanted around denuded root surfaces either autologously or allogeneically. A mixture of β-tricalcium phosphate and collagen gel was placed on the bone defects. Eight weeks after transplantation, dogs were euthanized and subjected to microcomputed tomography and histological analyses. RNA and DNA were extracted from the paraffin sections to verify the presence of EGFP at the transplantation site. Inflammatory markers from peripheral blood sera were quantified using an enzyme-linked immunosorbent assay. Periodontal regeneration was observed in both the autologous and the allogeneic transplantation groups. The allogeneic transplantation group showed particularly significant regeneration of newly formed cementum, which is critical for the periodontal regeneration. Serum levels of inflammatory markers from peripheral blood sera showed little difference between the autologous and allogeneic groups. EGFP amplicons were detectable in the paraffin sections of the allogeneic group. These results suggest that allogeneic PDL-MSC sheets promoted periodontal tissue regeneration without side effects. Therefore, allogeneic transplantation of PDL-MSC sheets has a potential to become an alternative strategy for periodontal regeneration.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Hajishengallis, G" uniqKey="Hajishengallis G">G Hajishengallis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lalla, E" uniqKey="Lalla E">E Lalla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lockhart, Pb" uniqKey="Lockhart P">PB Lockhart</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Koop, R" uniqKey="Koop R">R Koop</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ramseier, Ca" uniqKey="Ramseier C">CA Ramseier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Fm" uniqKey="Chen F">FM Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name 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N">N Keklikoglu</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Iwata, Takanori" sort="Iwata, Takanori" uniqKey="Iwata T" first="Takanori" last="Iwata">Takanori Iwata</name><name sortKey="Izumi, Yuichi" sort="Izumi, Yuichi" uniqKey="Izumi Y" first="Yuichi" last="Izumi">Yuichi Izumi</name><name sortKey="Kinoshita, Atsuhiro" sort="Kinoshita, Atsuhiro" uniqKey="Kinoshita A" first="Atsuhiro" last="Kinoshita">Atsuhiro Kinoshita</name><name sortKey="Okano, Teruo" sort="Okano, Teruo" uniqKey="Okano T" first="Teruo" last="Okano">Teruo Okano</name><name sortKey="Takagi, Ryo" sort="Takagi, Ryo" uniqKey="Takagi R" first="Ryo" last="Takagi">Ryo Takagi</name><name sortKey="Tsumanuma, Yuka" sort="Tsumanuma, Yuka" uniqKey="Tsumanuma Y" first="Yuka" last="Tsumanuma">Yuka Tsumanuma</name><name sortKey="Washio, Kaoru" sort="Washio, Kaoru" uniqKey="Washio K" first="Kaoru" last="Washio">Kaoru Washio</name><name sortKey="Yamada, Azusa" sort="Yamada, Azusa" uniqKey="Yamada A" first="Azusa" last="Yamada">Azusa Yamada</name><name sortKey="Yamato, Masayuki" sort="Yamato, Masayuki" uniqKey="Yamato M" first="Masayuki" last="Yamato">Masayuki Yamato</name><name sortKey="Yoshida, Toshiyuki" sort="Yoshida, Toshiyuki" uniqKey="Yoshida T" first="Toshiyuki" last="Yoshida">Toshiyuki Yoshida</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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